Protein_Domain

Part:BBa_K5319694

Designed by: Jiawei Wang   Group: iGEM24_ZJU-China   (2024-10-01)


creN1-59

The n-terminal truncate of the cre enzyme in a blue-induced cleavage recombinant enzyme system,with the split site at 59AA

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 808
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 808
    Illegal BglII site found at 76
    Illegal XhoI site found at 790
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 808
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None